Figure 2.

Efg1 regulates biofilm development by C. parapsilosis.A. The in vitro biofilm mass of smooth and concentric colonies from wild type (CLIB214), efg1Δ/efg1Δ deletion strains (including a mixture of strains LCP2, LCP5 and LCP8) and the reconstituted strain LCP7_RI was determined by measurement of dry weights. The average ± standard deviation from three independent measurements is shown. The panels below show biofilm growth on 24-well Nunc plates stained with crystal violet.B. In vivo biofilms were developed in the rat catheter model. Concentric wild-type (CLIB214) cells and smooth efg1/efg1 deletion (LCP1) cells were allowed to develop for 24 h and then visualized by SEM at two magnifications.