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Deleting Efg1 increases sensitivity to cell wall stress. Three microlitres from serial dilutions in PBS was inoculated on YPD alone, and YPD with SDS (0.04%), caspofungin (0.1 μg ml−1) and calcofluor white (12 μM). Strains include concentric (c) and smooth (s) colonies from wild type (C. parapsilosis CLIB214), efg1Δ/efg1Δ deletion (LCP8) and the reconstituted strain (LCP7_RI).
