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. 2014 Feb;99(2):267–275. doi: 10.3324/haematol.2013.090076

Figure 5.

Figure 5.

In vivo supplementation with resveratrol ameliorates β-thalassemic ineffective erythropoiesis, up-regulates Foxo3 and peroxiredoxin-2 (Prdx2). (A) Cytofluorimetric analysis of maturation pattern of wild-type (wt) and β-thalassemic (b-thal) erythroid precursors from the bone marrow of mice with or without resveratrol supplementation using the following surface markers: CD44 and TER119 (Online Supplementary Appendix and Figure S5B). This cytofluorimetric strategy allows the identification of the following homogenous cell populations: population I corresponding to pro-erythroblasts, population II corresponding to basophilic erythroblasts, population III corresponding to polychromatic erythroblasts and population IV corresponding to orthochromatic erythroblsts. Data presented as means ± SD (absolute cell counts are shown in Online Supplementary Figure S5C) (n=10); *P<0.05 compared to untreated mice. (B) (Left): RT-PCR expression of Foxo3 and peroxiredoxin-2 (Prdx2) on sorted basophilic erythroblasts from bone marrow of mice with or without resveratrol (Resv) supplementation. Sorted basophilic erythroblasts and polychromatic erythroblasts from 10 different mice from each mouse group were analyzed. Experiments were performed in triplicate. Error bars represent standard deviations (mean ± SD; *P<0.05 compared to untreated mice, n=10). (Right): RT-PCR expression of peroxiredoxin-2 (Prdx2) on sorted polychromatic erythroblasts from bone marrow of mice with or without resveratrol (Resv) supplementation. Sorted polychromatic erythroblasts from 10 different mice from each group were analyzed. Experiments were performed in triplicate. Error bars represent the standard deviations (mean ± SD; *P<0.05 compared to untreated mice; n=10). (D) Immunoblot analysis of PRDX2 in sorted basophilic and polychromatic erythroblasts of wild-type (wt) and β-thalassemic mice with or without resveratrol (Resv) supplementation. Band 3 was used as loading control. One representative experiment of 4 others with similar results. (Right): relative quantification of immunoreactivity of peroxiredoxin-2 (Prdx2) and band 3 in sorted polychromatic erythroblasts of wild-type (wt) and β-thalassemic (b-thal) mice with or without resveratrol (Resv) supplementation. Data expressed as Prdx2/band 3 ratio and presented as means ±SD (n=5); *P<0.05 compared to untreated mice.