Figure 3.

Enhanced BM engraftment with peripheral hematopoietic deficiencies. Secondary BM transplant was carried out by transfer of IEX-1 KO (KO) or WT BM cells (CD45.2) into lethally irradiated cognate PepJ mice (CD45.1) or PepJ BM cells reciprocally into WT or KO recipients. Peripheral (A) and BM (B) chimerism was analyzed 3 months later as percent donor cells based on CD45.1 versus CD45.2 expression. Platelet (C) and RBC (D) counts were evaluated in one (filled) and three months (unfilled) post-BM transplantation. A normal level of platelets and RBCs in the periphery is indicated by a horizontal dash level. Data are presented as mean ± SD, n= 5 mice per group. **, P<0.01, compared in PepJ recipients receiving WT vs. KO donor BM cells.