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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Pharmacol Biochem Behav. 2013 Nov 23;116:142–151. doi: 10.1016/j.pbb.2013.11.021

Figure 4.

Figure 4

Adolescent intermittent ethanol (AIE) binge caused an increase in Wisteria floribunda agglutinin (WFA) immunoreactivity (IR) in the adult orbitofrontal cortex (OFC). Mice were given either water or ethanol (5g/kg, i.g.) in an intermittent fashion (2 days on − 2 days off) during adolescence (P28–37). Immunohistochemistry was performed following sacrifice in adulthood (P110). (A) Representative images of WFA-IR in the OFC of control and AIE groups in adulthood show increased WFA-IR neural nets and increased intensity of staining in the AIE group. (B) Quantification of WFA-IR showed a significant increase in WFA-IR *p<0.05, t-test. N = 5 Control, 5 AIE. Scale bare denotes 50 microns.