Adolescent intermittent ethanol (AIE) binge did not cause any change in phosphocan, HABP, or tensacin-C immunoreactivity (IR) in the adult orbitofrontal cortex (OFC). Mice were given either water or ethanol (5g/kg, i.g.) in an intermittent fashion (2 days on − 2 days off) during adolescence (P28–37). Immunohistochemistry was performed following sacrifice in adulthood (P110). (A) Quantification of phosphocan-IR showed no change between control and AIE groups p=0.94. (B) Quantification of HABP-IR showed no change between control and AIE groups p=0.88. (C) Quantification of Tensacin-C-IR showed no change between control and AIE groups p=0.33, t-test. N = 5 Control, 6 AIE.