Table 1.
Effect of sequential addition of TNF-α and IFN-γ on CCL5 release by HPFB.
| Stimulus 1 | Stimulus 2 | CCL5 (pg/104 cells) |
|---|---|---|
| Medium | Medium | Undetectable |
| Medium | IFN-γ | Undetectable |
| Medium | TNF-α | 4 ± 2 |
| Medium | TNF-α + IFN-γ | 24 ± 13 |
| IFN-γ | Medium | 1 ± 1 |
| IFN-γ | TNF-α | 9 ± 4 |
| TNF-α | Medium | 6 ± 4 |
| TNF-α | IFN-γ | 9 ± 7 |
| TNF-α + IFN-γ | Medium | 146 ± 20 |
Cells were incubated with TNF-α (1000 pg/mL) and/or IFN-γ (25 U/mL) for 24 hours (stimulus 1), washed, and incubated again for the next 24 hours in the presence or absence of these cytokines (stimulus 2). Data were derived from two independent experiments.