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. 2014 Jan 9;2014:601383. doi: 10.1155/2014/601383

Figure 7.

Figure 7

The effects of ECD dietary supplementation on apoptosis and necrosis of lymphocytes in aged SAM-P8 mice. Eight-month-old male SAM-P8 and control SAM-R1 mice were divided into 5 groups. The 3 treatment groups of SAM-P8 animals were fed for four weeks with diets supplemented with low (100 mg/kg), medium (500 mg/kg), and high (2500 mg/kg) doses of ECD and the two control animal groups were fed on the same diet without supplementation. Following fasting for 12 hours, peripheral blood was collected from anesthetized animals and subjected to FACS analysis all as described in Section 2. (a) Representative FACS plots of the different groups of mice generated by gating on double stained blood lymphocytes with Annexin V-FITC/PI. (b) Histogram showing the percentage of apoptotic lymphocytes (Annexin V-FITC positive cells) in peripheral blood of the different animal groups. (c) Histogram showing the percentage of necrotic lymphocytes (PI positive cells) in peripheral blood of the different animal groups. Bars represent the mean ± SD (in each group, n = 10).# P < 0.05 SAM-P8 versus SAM-R1; # P < 0.05 each treated group versus SAM-P8 group (by one way ANOVA with post hoc test).