Figure 8.

Effects of the ECD dietary supplementation on serum proinflammatory cytokine IL-6 level of SAM-P8 mice. Eight-month-old male SAM-P8 and control SAM-R1 mice were divided into 5 groups. The 3 treatment groups of SAM-P8 animals were fed for four weeks with diets supplemented with low (100 mg/kg), medium (500 mg/kg), and high (2500 mg/kg) doses of ECD and the two control animal groups were fed on the same diet without supplementation. Following fasting for 12 hours, the plasmas were collected and plasma cytokines in the different animal groups were determined by cytometric bead array immunoassay all as described in Section 2. (a) Representative FACS plots of the different groups of mice generated by gating on IL-6 cytometric bead array. (b) Histogram showing the concentrations of cytokine IL-6 in plasma of the different animal groups. Bars represent the mean ± SD (in each group, n = 10). *P < 0.001 each treated group versus SAM-P8 group (by one way ANOVA with post hoc test).