Table 1.
EC50s and IC50s for described AgrC variants.
| WT AgrC | AgrCHis (H239Q) | AgrCKin (G394A, G396A) | AIP-I EC50 (nM), (95% CI) | AIP-II EC50 (nM), (95% CI) | AIP-III IC50 (nM), (95% CI) |
|---|---|---|---|---|---|
| AgrC-I | 10, (8.7-13) | - | |||
| AgrC-I | - | - | |||
| AgrC-I | - | - | |||
| AgrC-I | AgrC-I | 40, (35-45) | - | 18, (13-25)* | |
| AgrC-I-GFP | 16, (14-17) | - | |||
| AgrC-I-GFP | - | - | |||
| AgrC-I-GFP | - | - | |||
| AgrC-I-GFP | AgrC-I-GFP | 36, (31-42) | - | ||
| AgrC-ISensor | - | - | |||
| AgrC-ISensor | - | - | |||
| AgrC-ISensor | AgrC-ISensor | 6100, (4200-9000) | - | ||
| AgrC-I | AgrC-ISensor | 200, (170-240) | - | 140, (88-230)# | |
| AgrC-ISensor | AgrC-I | 310, (260-380) | - | 320, (150-650)# | |
| AgrC-II | - | 2.9, (2.3-3.6) | |||
| AgrC-II | - | - | |||
| AgrC-II | - | - | |||
| AgrC-I | AgrC-II | 21, (18-25) | 17, (13-22) | 15, (9.8-21)* | |
| AgrC-II | AgrC-I | 17, (16-19) | 4.5, (3.6-5.6) | 17, (13-22)* |
“-” indicates no activation by any AIP concentration tested.
*
150 nM AIP-I added.
#
1 μM AIP-I added.
Parentheses following an EC50 or IC 50 value denote the 95 percent confidence interval.