Table 5.

Experimental design and results using face-centered full factorial design (FCCD).

Run	Skim milk, x 1		Yeast extract, x 2		Responsesa
	Coded value	Actual value	Coded value	Actual value	Max. biomass, y 1 (log10 cfu/mL)	β-Galactosidase production y 2 (U/mL)	Lactose balance y 3 (g/L)	FAN balance y 4 (mg/L)
1	−1	4.0	−1	1.0	5.15	0.19	4.59	198.24
2	−1	4.0	0	1.9	5.18	3.03	4.51	209.58
3	−1	4.0	+1	2.8	5.22	3.13	4.40	213.24
4	0	6.0	−1	1.0	7.09	7.06	10.83	229.15
5	0	6.0	0	1.9	7.15	8.37	11.53	237.06
6	0	6.0	0	1.9	7.11	9.40	11.58	231.04
7	0	6.0	0	1.9	7.12	9.32	11.81	234.65
8	0	6.0	0	1.9	7.14	8.35	11.91	238.98
9	0	6.0	0	1.9	7.16	9.38	11.84	233.14
10	0	6.0	+1	2.8	7.13	8.41	12.90	359.76
11	+1	8.0	−1	1.0	7.26	7.02	22.41	287.53
12	+1	8.0	0	1.9	7.23	6.27	19.10	290.59
13	+1	8.0	+1	2.8	7.11	6.18	25.25	318.70

^aAll factorial and axial points are means of duplicate.