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. 2014 Jan 21;14:6. doi: 10.1186/1472-6750-14-6

Table 1.

Composition of media used in bioreactor cultivations

 
 Auto-induction
 Complex media
 Defined media
Nutrient (g L -1 ) Batch Pulse Batch Fed-batch Batch Fed-batch
Glucose
 10.0
 ---
 10.0
 ---
 ---
 ---
Glycerol
 60.0
 258.3
 40.0
 800
 40.0
 800.0
Lactose
 20
 130.0
 ---
 ---
 ---
 ---
Tryptone
 10.0
 10.0
 10.0
 10.0
 ---
 ---
Yeast extract
 5.0
 5.0
 5.0
 5.0
 ---
 ---
MgSO4.7H2O
 0.5
 0.5
 0.5
 40.0
 1.6
 20.0
KH2PO4
 3.4
 3.4
 3.4
 3.4
 17.7
 21.28
(NH4)2HPO4
 ---
 ---
 ---
 ---
 5.3
 6.4
Na2HPO4.12H2O
 9.0
 9.0
 9.0
 9.0
 ---
 ---
Citric acid
 ---
 ---
 ---
 ---
 2.27
 2.27
NH4Cl
 2.7
 2.7
 2.7
 2.7
 ---
 ---
Na2SO4
 0.7
 0.7
 0.7
 0.7
 ---
 ---
Thiamine --- --- --- --- 45.0 45.0

Auto-induction medium: cultivation B1; complex medium: cultivation B2; defined medium: cultivation B3.