Table 4.
PGA production in various E. coli expression systems for bioreactor cultures carried out under different induction strategies
| E. coli strain/construction | Ind. | Op. mode | T ind (°C) | [Biomass] (gDCW L -1 ) | EA sp (IU DCW -1 ) | Vol. Enz.Act. (IU mL -1 ) | Product. (IU mL -1 h -1 ) | Ref. |
|---|---|---|---|---|---|---|---|---|
| RE3/ pKA18 |
Phenyl acetic acid |
Batch |
28 |
5 |
1000 |
4.5 |
0.28* |
[33] |
| MDDP7/ pTrcKnPAC2902 |
IPTG |
Fed-batch |
28 |
33 |
1020* |
37.5 |
0.48* |
[6] |
| W3110/pPA102 |
Cheese whey |
Batch |
29 |
1.5 |
781 |
0.901 |
0.053* |
[23] |
| 9633/pGL-5 |
IPTG |
Fed-batch |
30 |
96.9* |
157* |
15 |
0.63* |
[24] |
| BL21(DE3)/pET28b |
IPTG |
Batch |
28 |
12 |
800 |
10 |
0.42* |
[21] |
| DH5α/ Psmlfpga |
IPTG |
Batch |
30 |
21.7* |
153 mg gDCW-1 |
79.88 |
0.67* |
[22] |
| DH5α/pET30b |
Constitutive |
Batch |
28.5 |
4.5 |
NA |
43 |
2.58* |
[20] |
| BL21(DE3)/pT101/D-TOPO |
Lactose |
Continuous fed-batch |
20 |
100 |
2000 |
190 |
7.8 |
This work |
| BL21(DE3)/pT101/D-TOPO | Lactose | Intermittent fed-batch | 20 | 38 | 2800 | 94 | 3.9 | This work |
*Estimated from reported data.
NR Not reported.