Fig. 7.

Growth inhibition by EC0905 or DAVLBH in the presence or absence of 20 µM folic acid. (A) Growth inhibition by EC0905 in the absence of 20 µM folic acid. (B) Growth inhibition by EC0905 in the presence of 20 µM folic acid. (C) Growth inhibition by DAVLBH in the absence of 20 µM folic acid. (D) Growth inhibition by DAVLBH in the presence of 20 µM folic acid. M160-8, PCFT-h and PCFT-4 cells were detached from culture plates with 0.5 mM EDTA in phosphate-buffered saline and seeded in 96-well plates (0.1 ml) at a density of 2000 cells/well. An equal volume of drug solutions in the presence or absence of folic acid, diluted in the growth medium, was added to cells the next day, after which the cells were grown for an additional 5 days. Data are the mean ± S.E.M. from three independent experiments.