TABLE 1.

Relative levels of PCFT and FR mRNA in PCFT-4, M160-8, PCFT-h, and R1-11 cells determined by real-time PCR

The mRNA levels of both PCFT and FR in PCFT-4 cells are arbitrarily set to 1. Data are mean ± S.E. from the analysis of samples collected in three experiments performed on three separate days. The PCFT mRNA values in M160-8 and R1-11 cells are not different. The FR mRNA levels in PCFT-4, PCFT-h, and R1-11 cells are not different from each other. Using subtype-specific primers, it was determined that FR-α, not FR-β, is overexpressed in M160-8 cells.

	PCFT	FR
R1-11-PCFT-4	1	1
M160-8	0.018 ± 0.02	8.3 ± 2.6
R1-11-PCFT-h	132 ± 19	1.04 ± 0.49
R1-11	0.0087 ± 0.003	1.41 ± 0.62