Fig. 4.

H/R-induced apoptosis is attenuated by RSK1 inhibitor SL0101. ARVMs (A) or HL-1 cells (B) were treated with or without SL0101 (50 μM) and exposed to normoxia or hypoxia (1% O₂) for 24 hours, followed by reoxygenation for 1 hour. Cell lysates were subjected to Western analysis. Panels with bar graphs are quantitative data from Western blots of five (for ARVMs) or four (for HL-1) different experiments normalized for actin (loading control). Data shown are means ± S.E.M. *P < 0.05. Ctr, control; N, normoxia.