Table 5.
Enzyme activities, ADP-glucose and starch contents in NPP1-overexpressing rice suspension-cultured cells
| Enzyme activities (mU mg−1) | WT | UNP1-1 | UNP1-2 | UNP1-3 |
|---|---|---|---|---|
| AGPPase | 4.0 ± 0.3 | 204.4 ± 18.1 | 158.1 ± 12.4 | 113.1 ± 8.7 |
| AGPase | 126.2 ± 22.8 | 109.4 ± 11.7 | 96.1 ± 19.7 | 110.9 ± 12.5 |
| SuSy | 414.8 ± 25.6 | 387.2 ± 25.3 | 407.4 ± 45.3 | 257.5 ± 19.6 |
| UGPase | 206.7 ± 10.6 | 175.5 ± 14.3 | 233.1 ± 7.1 | 266.2 ± 15.0 |
| Phosphoglucomutase | 184.5 ± 5.0 | 167.1 ± 12.9 | 178.5 ± 17.5 | 176.9 ± 7.8 |
| Phosphoglucoisomerase | 129.1 ± 10.4 | 122.5 ± 16.4 | 125.5 ± 29.0 | 123.8 ± 9.8 |
| Starch phosphorylase | 102.8 ± 4.1 | 80.3 ± 5.1 | 97.6 ± 5.4 | 95.3 ± 10.5 |
| Hexokinase | 62.3 ± 5.5 | 78.6 ± 4.8 | 79.6 ± 12.3 | 69.1 ± 4.2 |
| Soluble SS | 50.4 ± 5.4 | 153.9 ± 17.7 | 151.6 ± 7.3 | 219.7 ± 33.9 |
| ADP-glucose (nmol g−1 FW) | 3.23 ± 0.21 | 1.02 ± 0.40 | 1.21 ± 0.13 | 1.51 ± 0.03 |
| Starch contents (µmol glucose g−1 FW) | 12.02 ± 0.23 | 5.35 ± 0.04 | 4.63 ± 0.04 | 3.52 ± 0.01 |
Suspension-cultured cells of WT and of three independent NPP1-overexpressing UNP1 lines were harvested after 7 d of culture and subjected to assays.
Values show the means ± sa (n = 3). mU mg−1, nmol min−1 (mg of protein)−1.