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. 2014 Jan 30;55(2):251–257. doi: 10.1093/pcp/pcu003

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© The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — CO₂ permeability of PIP2 aquaporins of barley. (A) Representative traces of cytosolic pH change of water-, HvPIP2;1 cRNA-, HvPIP2;3 cRNA- and HvPIP2;4 cRNA-injected X. laevis oocytes. cRNAs and carbonic anhydrase were injected 24–48 h before the measurements. Ticks above the traces indicate where the bath solutions were replaced with the modified Barth’s solution containing the designated concentrations of CO₂/H₂CO₃. (B) P_CO2 of the cell membrane of X. laevis oocytes injected with water (n = 14), HvPIP2;1 cRNA (n = 10), HvPIP2;2 cRNA (n = 5), HvPIP2;3 cRNA (n = 6), HvPIP2;4 cRNA (n = 7) or HvPIP2;5 cRNA (n = 3). Error bars indicate the SEM. Asterisks indicate significant difference of the mean from the water-injected control (Water) by Student’s t-test at α = 0.05.