Fig. 2.

Short-term responses of A_sat, intercellular [CO₂] and leaf temperature measured under a CO₂ concentration ([CO₂]-M) of (a) 380 and (b) 580 µmol mol⁻¹ at mid-grain filling. Plants were grown at elevated (FACE) or ambient (Amb) [CO₂] and elevated (ET) or normal (NT) soil and water temperatures. A_sat, intercellular [CO₂] and leaf temperature were measured at the same CO₂ concentrations ([CO₂]-M) regardless of CO₂ concentrations during plant growth. For significant results, ANOVA P-values are indicated in each panel (*P < 0.05, n.s., not significant): year as the main plot, growth [CO₂] as the split plot, and soil and water temperatures (T) as the split–split plot. Values are the means ± SE (n = 3). No interaction effects were significant.