Skip to main content
. 2014 Feb 4;9(2):e86184. doi: 10.1371/journal.pone.0086184

Figure 1. Influence of the improvements of the B-cell handling and the B-cell cultivation.

Figure 1

(A) Percentage of IgG-producing B-cell clones per total wells and (B) average IgG concentration over all IgG-positive wells after +/− pre-incubation in medium and +/− centrifugation post sorting. For each parameter 368 wells were analyzed. (C) Percentage of IgG-producing B-cell clones (ASCs) per total wells and (D) average IgG concentration over all IgG-positive wells after using +/− SAC in a dilution of 1∶20000 during B-cell cultivation. For each parameter 252 wells were analyzed. (E) Percentage of IgG-producing B-cell clones per total wells, (F) average IgG concentration over all IgG-positive wells, as well as percentage of antigen specific B-cell clones (G) per total wells and (H) per IgG-producing B-cell clones after +/− protein panning. For each parameter around 3500 wells were analyzed. The error bars represent the standard deviation. The cut off value of IgG-positive wells was >0.013 µg/ml IgG and of human IL1RL1-positive wells was>OD 0.195.