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. 2014 Feb 4;9(2):e87342. doi: 10.1371/journal.pone.0087342

Figure 4. Analysis of hCAR expression and cell killing activity of HAdV-D9 in cancer cell lines.

Figure 4

(A) Analysis of hCAR expression in various cancer cell lines by flow cytometry. Filled, gray histograms indicate stained cells; open, white histograms indicate unstained control cells. (B) Two-dimensional cell viability assay. Cancer cell lines were infected with HAdV-C5 (black squares) or HAdV-D9 (white squares) at indicated MOIs. Cell survival in each well was measured at 6 days post-infection using an MTS assay and plotted on y-axis as the percentage of the control values obtained from uninfected cells. (C) Three-dimensional cell viability assay. Spheroids were infected with HAdV-C5 (black squares) or HAdV-D9 (white squares) at indicated MOIs. Cell survival in each well was measured at 12 days post-infection using an MTT assay and plotted on y-axis as the percentage of the control values obtained from uninfected cells. Data points represent mean+standard error of the mean (n = 3).