Skip to main content
. 2014 Feb 4;9(2):e87220. doi: 10.1371/journal.pone.0087220

Figure 1. Phosphorylation and mutational status of genes that encode components of the RTK/Ras/PI3K pathway.

Figure 1

Nine ovarian clear cell adenocarcinoma (OCCA) and a control (Cntl) cell line (immortalized epithelial cells from ovarian endometrioma) were lysed in cell lysis buffer and analyzed by western blotting. In general, most of the OCCA cell lines displayed higher levels of phosphorylation of Akt (Thr308) and its downstream targets (GSK3β, FOXO 1/3a, 4EBP1 and S6) than the respective levels of phosphorylation in the control line. The abundances and levels of phosphorylation of c-MET (Tyr1234/1235), HER2 (Tyr1221/1222), and HER3 (Tyr1289) were also evaluated. The mutational status of PIK3CA, PTEN, and K-Ras is shown for each cell line.