Table 2. Expression analyses of MUG-Chor1 candidate genes in U-CH1 cells.
| Gene | TaqMan GeneExpression Assay | Mean Cq andstandard deviationa | Fold changeb | p-value | |
| small cells | large cell | large vs. small | |||
| ALG11 | Hs01076287_m1 | 32.5±4.1 | 29.3±0.8 | 10.6 | 0.030 |
| UCHL3 | Hs00234683_m1 | 29.6±1.4 | 27.9±0.8 | 3.7 | 2.3•10−4 |
| TMEM144 | Hs00938021_m1 | 32.4±2.9 | 31.5±1.6 | 2.1 | 0.26 |
| PPP2CB | Hs00602137_m1 | 33.4±4.5 | 31.3±1.5 | 4.9 | 0.026 |
| ACTB | Hs01060665_g1 | 27.4±2.3 | 27.3±1.0 | – | – |
| GAPDH | Hs02758991_g1 | 27.4±2.9 | 27.0±1.1 | – | – |
RT-qPCR was done on AB7900 TaqMan (Applied Biosystems; Foster City, CA). Normalization (GAPDH and ACTB) and statistical analysis was done with GenEx Professional (MultiD Analysis; Version 5.3.5.6; see also 2.7). Cut-off for multiple testing (ALG11, UCHL3, TMEM144 and PPP2CB) was p = 0.01274.
a
calculated as mean values from quadruplicate or triplicate (in case the Cq value could not be defined) biological samples.
b
Cq values were normalized to GAPDH and ACTB (ΔCq). Differential expression (ΔΔCq) is given as positive (up-regulated in large cells) or negative (down-regulated in large cells) fold change ( = 2ΔΔCq).