Figure 3. flgZ is implicated in the regulation of swimming motility.

A. Relative swimming motility of flgZ mutants in P. fluorescens F113 and P. putida KT2440 backgrounds. B. Relative swimming motility of P. fluorescens F113 derivatives harboring plasmids which overexpress the F113 (pflgZ F113) and KT2440 (pflgZ 2440) flgZ genes. C. Relative swimming motility of P. putida KT2440 derivatives harboring plasmids which overexpress the F113 (pflgZ F113) and KT2440 (pflgZ 2440) flgZ genes. In B and C wild-type strains harbored the empty vector pVLT31 and flgZ expression was induced with 1mM IPTG. Swimming motility was determined by haloes diameter in SA plates with 0.3% agar, 24 hours after inoculation. Averages of three triplicated experiments plus standard deviation are shown. Different letters indicate statistically significant differences (p<0.05). Relative motility of each strain is also shown in Table S3.