Table 1. V1V2 scaffold antigens used to study the reactivity of plasma specimens from RV144 vaccine and placebo recipients.

V1V2-scaffold Antigens	Phase 1+	Phase 2‡	Case-Control
			ELISA*	BAMA**
gp70WT.AP		1∶100§
gp70WT.LL	+
gp70.B(Case A2.p623)-V1V2.APorig		1∶100	1∶100	1∶100
gp70.B(Case A2.p623)-V1V2.AP(new batch)		1∶100
J08.C(97ZA012)-V1V2	+
J08.B(HxB2)-V1V2	+
J08.A(92RW020)-V1V2	+
tags.B(Case A2)-V1V2	+
tags.B(63521)-V1V2	+
gp70.B(Case A2)-V1V2.LL	+	1∶100	1∶100	1∶100
gp70.A(Q23)-V1V2	+
gp70.C(97ZA012)-V1V2	+	1∶100	1∶100	1∶100
gp70.B(Case A2)-V1V2		1∶100
gp70.B(HxB2)-V1V2	+	1∶100
gp70.B(Case A2.p565f pur)-V1V2	+	1∶100
tags.A(Q23)-V1V2	+	1∶300
gp70.A(92RW020)-V1V2	+	1∶100	1∶100	1∶100
tags.A(9004SS)-V1V2	+	1∶100
tags.D(9009SA)-V1V2	+
tags.AE(92TH023)-V1V2	+
tags.AE(A244/D7N)-V1V2	+
tags.C(1086)-V1V2	+	1∶300	1∶300	1∶100
tags.AE(A244)-V1V2	+	1∶100
gp70.AE(92TH023)-V1V2	+	1∶900	1∶900	1∶100

⁺indicates six 3-fold serial plasma dilutions starting with 1∶100 used in Phase 1.

^‡indicates plasma dilution used in Phase 2 and case-control experiments.

^*ELISA = enzyme-linked immunosorbent assay;

^**BAMA = binding antibody multiplex assay;

^§Plasma dilutions used in assays.