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. 2014 Feb 4;9(2):e87772. doi: 10.1371/journal.pone.0087772

Table 1. Shows the list of different antibody combinations according to the cell development stages in the various lymphoid organs.

Cell Development Developmental stage Antibody combination
B cell development (BM) Total B cells CD19+B220+
Pro-B cells CD43+CD2IgMIgD
Pre-B cells CD43CD2+IgMIgD
Immature B cells IgM+IgDlow
Recirculating mature B cells IgM+/lowIgDhigh
B cell lymphoid population (Spleen) Total B cells CD19+B220+
Pro-pre B cells IgMlowIgDlow
Immature B cells IgM+IgD
Mature B cells IgM+IgD+
Marginal zone B cells CD21+CD23
Follicular zone B cells CD21CD23+
T cell development (Thymus) Double Negative stages (DN) CD3CD4CD8
DN1 CD44+CD25
DN2 CD44+CD25+
DN3 CD44CD25+
DN4 CD44CD25
Immature Single Positive (ISP) CD8+CD3CD69
Double Positive (DP) CD3−/lowCD4+CD8+
CD8+ Single Positive (SP) CD3+CD8+CD4
CD4+ Single Positive (SP) CD3+CD4+CD8
T cell lymphoid population (Spleen) Total T cell population CD3+NK1.1
CD3+CD4+ SP CD3+CD4+CD8
Memory T cells CD4+CD8CD62Llow
Naïve T cells CD4+CD8CD62L+CD25
Naïve Tregs CD4+CD8CD62L+CD25+