Table 1. Shows the list of different antibody combinations according to the cell development stages in the various lymphoid organs.
Cell Development | Developmental stage | Antibody combination |
B cell development (BM) | Total B cells | CD19+B220+ |
Pro-B cells | CD43+CD2−IgM−IgD− | |
Pre-B cells | CD43−CD2+IgM−IgD− | |
Immature B cells | IgM+IgDlow | |
Recirculating mature B cells | IgM+/lowIgDhigh | |
B cell lymphoid population (Spleen) | Total B cells | CD19+B220+ |
Pro-pre B cells | IgMlowIgDlow | |
Immature B cells | IgM+IgD− | |
Mature B cells | IgM+IgD+ | |
Marginal zone B cells | CD21+CD23− | |
Follicular zone B cells | CD21−CD23+ | |
T cell development (Thymus) | Double Negative stages (DN) | CD3−CD4−CD8− |
DN1 | CD44+CD25− | |
DN2 | CD44+CD25+ | |
DN3 | CD44−CD25+ | |
DN4 | CD44−CD25− | |
Immature Single Positive (ISP) | CD8+CD3−CD69− | |
Double Positive (DP) | CD3−/lowCD4+CD8+ | |
CD8+ Single Positive (SP) | CD3+CD8+CD4− | |
CD4+ Single Positive (SP) | CD3+CD4+CD8− | |
T cell lymphoid population (Spleen) | Total T cell population | CD3+NK1.1− |
CD3+CD4+ SP | CD3+CD4+CD8− | |
Memory T cells | CD4+CD8−CD62Llow | |
Naïve T cells | CD4+CD8−CD62L+CD25− | |
Naïve Tregs | CD4+CD8−CD62L+CD25+ |