Table 2. Interaction parameters of RNase A with the α-hemolysin pore under various experimental conditions.
| Parametera | RNase A | RNase A (purified) | RNase A+TCEP | RNase A+GdnHCl | RNase A+TCEP+GdnHCl | Unfolded RNase A |
| I1 (pA)b | −26.3 | −25.4 | −26.3 | −25.8 | −25.9 | −25.7 |
| I2 (pA) | −70.0 | −75.5 | −68.3 | −90.7 | −92.4 | −49.5 |
| I3 (pA) | – | – | – | – | – | −71.6 |
| T1 (ms)c | 0.02 | 0.05 | 0.03 | 0.08 | 0.04 | 0.16 |
| T2 (ms) | 0.07 | 0.11 | 0.11 | 0.14 | 0.10 | 0.21 |
| T3 (ms) | – | – | – | – | – | 0.19 |
| A1 (%)d | 37.2 | 39.7 | 31.5 | 13.28 | 17.3 | 23.5 |
| A2 (%) | 62.8 | 60.3 | 68.5 | 86.72 | 82.7 | 35.2 |
| A3 (%) | – | – | – | – | – | 41.3 |
a
I1, I2, I3, T1, T2, and T3 represent the amplitudes and the durations of the current blockades of the respective event populations presented in Figure 3. A1, A2, and A3 are the percent of total events forming each respective population. The peaks are numbered from right to left. A dash indicates the absence of third event population.
b
The error is estimated to be ±1 pA.
c
The error is estimated to be ±10%.
d
The error is estimated to be ±1%.