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. 2014 Feb 4;9(2):e87998. doi: 10.1371/journal.pone.0087998

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© 2014 Yu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Westernblotting shows protein levels of exogenous and endogenous mdig (pointed by red arrows) in the cells stably transfected with mdig-GFP or shRNA that specifically silences mdig expression. B. MTT-based cell proliferation assay indicates that mdig overexpression enhances cell proliferation. Five independent stably mdig-transfected clones were analyzed. C. Silencing mdig by two individual shRNAs that target different region of the mdig mRNA suppressed cell proliferation. Ctrl: control; shR: shRNA.