Figure 2. Effect of of anti-catalytic mAbs to N-domain active center for A and C for B) and from patient # 27 (arrowed in Fig. 1 C) were incubated with mAbs which are anti-catalytic for the N-domain active center - i2H5 and 3A5 (10 µg/ml)[23], [34].

Residual ACE activity was determined with two ACE substrates as in Fig. 1. We also measured ZPHL/HHL ratio-as in [20] and in Fig. 1 C. Bars highlighted with yellow - samples with values of ACE activity lower than mean ± 2SD for control sample without mAbs. Bars highlighted with red- samples with values of ZPHL/HHL ratio higher than mean ± 2SD for control samples without mAbs. Data presented are mean ± SD of 2–3 independent experiments in duplicates). * p<0.05 vs. control serum.