Figure 1. Administration of lead acetate does not cause damage to kidney tissue.

Young and old mice were injected with 10/100 g body weight and sacrificed 36 hours later; alternatively mice underwent kidney ischemia/reperfusion injury by clamping of the renal pedicles and were sacrificed 24 hours thereafter. (A) Haemotoxylin-eosin staining of kidney sections from young and old mice with or without lead acetate treatment show no difference in renal microstructure (G represents glomerulus, T represents tubule); original magnification 400×. Quantitative PCR for damage markers (B) Kim-1 and (C) NGAL in control young and old mice as well as young and old mice exposed to lead acetate or after IR damage. (D) LTL damage score of young and old mice injected with lead acetate shows no difference in brush border damage. (E) Quantification of cleaved caspase 3 positive cells; n = 5, data are mean values ± SEM. ***P<0.001