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. 2014 Feb 4;9(2):e88071. doi: 10.1371/journal.pone.0088071

Figure 5. In vitro culturing of primary tubular epithelial cells (PTEC) induces SCS-associated changes.

Figure 5

PTEC were isolated from young and old mice and harvested on day 0, day 3, or day 6 of culture. Quantitative PCR for (A) p16INK4a (B) p15INK4b, and (C) p19ARF in PTEC. (D) Quantification of SA-β-GAL staining on day 3 and 6 of PTEC culture. (E) Quantification of cells stained positive for BrdU on day 3 and 6 of PTEC culture. (F) BrdU uptake after lead acetate treatment in PTEC from young and old mice on day 6 of culture. n =  at least 4 separate mice, data are mean values ± SEM. *P<0.05; **P<0.01; ***P<0.001.