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. 2014 Feb 4;9(2):e88026. doi: 10.1371/journal.pone.0088026

Figure 1. Acute cocaine administration decreased pAkt-Thr308 in the caudate putamen of mice.

Figure 1

Levels of pAkt-Thr308 and pAkt-Ser473 were measured by Western blot analysis in mouse caudate putamen, nucleus accumbens and frontal cortex obtained 30 minutes following a single injection of cocaine (20 mg/kg). (1A) Cocaine significantly decreased pAkt-Thr308 (***p<0.001) and (1B) had no effect on pAkt-Ser473 in the caudate putamen. (1C&D) Levels of pAkt-Thr308 and pAkt-Ser473 were not changed in the nucleus accumbens or (1E&F) frontal cortex after cocaine administration. Bars represent the mean ± SEM (N = 7–13/group) and are expressed as a ratio of pAkt:total Akt. Data were analyzed by an unpaired two-tailed Student t-test. (1G) Representative immunoblots of caudate putamen tissue from saline (sal) and cocaine (coc) injected mice. Bands represent pAkt-Thr308, pAkt-Ser473, total Akt, and tubulin (from top to bottom).