Figure 5. The lux/ΔthrL and lux/ΔhyfC strains exhibit improvements over the parental lux/BW25113.

Each strain was propagated (N = 6) for 24 hours in M9 medium supplemented with ampicillin and IPTG in a Biotek Synergy2 plate reader (37°C, medium shaking); the OD₆₀₀ and luminescence were recorded from each culture every 15 minutes. The lux/ΔthrL strain (blue squares, A) grows more quickly and to higher cell density than does the lux/BW25113 (green squares), and produces more light (B). The lux/ΔhyfC transformant grows more slowly the parental control (C), but produces more light (D).