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. 2014 Feb 4;9(2):e88097. doi: 10.1371/journal.pone.0088097

Figure 3. Diagram depicting deletion mutations in the stem-loop and U-stretch of the trp attenuator.

Figure 3

The RNA segment that comprises the trp attenuator stem-loop is highlighted by a grey box with arrows indicating the complementary residues that form of the base-paired stem. Residues in the U-stretch of the attenuator are underlined. The sequences in bold show the G+C substitutions made in the regions downstream of the attenuator. The two most downstream of the 11 (G/U)AG triplet repeats (10 and 11) of the TRAP binding site are circled. The TRAP binding site was not altered by any of these deletion mutations. Numbers adjacent to structure names indicate fold TRAP regulation observed in vivo using β-galactosidase assays (results in Table 2).