| Primary antibodies |
| Anti SCP3 |
Rabbit polyclonal |
Abcam |
ab15093 |
1∶300 |
| Anti H3K9me3 |
Mouse monoclonal |
Abcam |
ab6001 |
1∶200 |
| Anti H3K9me3 |
Rabbit polyclonal |
Upstate (Millipore) |
07-442 |
1∶200 |
| Anti H3K27me3 |
Rabbit polyclonal |
Upstate (Millipore) |
05-851 |
1∶500 |
| Anti HP1 γ |
Mouse monoclonal |
Upstate (Millipore) |
05-689 |
1∶300 |
| AntiCBX1/HP1β |
Rat monoclonal |
Abcam |
ab10811 |
1∶5 |
| Anti ubiquitin-protein conjugates (FK2) |
Mouse monoclonal |
BIOMOL International, LP |
PW 8810 |
1∶300 |
| Anti H4K20me3 |
Rabbit polyclonal |
Abcam |
ab9053 |
1∶500 |
| Secondary antibodies |
| Alexa Fluor 568 goat anti mouse IgG (H+L) highly cross adsorbed |
Made in goat |
Molecular Probe (Invitrogen) |
A11031 |
1∶400 |
| Alexa Fluor 488 goat anti mouse IgG (H+L) highly cross adsorbed |
Made in goat |
Molecular Probe (Invitrogen) |
A11029 |
1∶400 |
| Alexa Fluor 568 goat anti rabbit IgG (H+L) |
Made in goat |
Molecular Probe (Invitrogen) |
A11011 |
1∶400 |
| Alexa Fluor 488 goat anti rabbit IgG (H+L) |
Made in goat |
Molecular Probe (Invitrogen) |
A11008 |
1∶400 |
| Alexa Fluor 350 goat anti mouse IgG (H+L |
Made in goat |
Molecular Probe (Invitrogen) |
A11045 |
1∶400 |
