Table 4.

Trace element analysis of cells cultured in the presence or absence of iron supplementation^*.

Sample	Iron#	Zinc#	Fe/Zn	n
A	0.047 ± 0.006	0.249 ± 0.047	0.19	7
A + Fe	0.667 ± 0.111•	0.117 ± 0.008°	5.70	7
F	0.044 ± 0.008	0.183 ± 0.034	0.24	5
F + Fe	0.650 ± 0.113•	0.139 ± 0.015°	4.68	4

Data were collected using either ICP–AES or –MS.

^*Cells were incubated in the presence (+Fe) or absence of 250 μM ferric nitrate.

^#Elemental analysis is reported as μg/mg protein; mean ± SEM.

^•Samples +/- Fe were evaluated using the Student’s t-test and showed significance at p < 0.01.

^°Samples +/- Fe were evaluated using the Student’s t-test and were not significant: p > 0.01.