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. 2014 Feb 5;34(6):2231–2243. doi: 10.1523/JNEUROSCI.1619-13.2014

Figure 5.

Figure 5.

The activated microglia raised the cytokine levels in the SVZ. A, A subpopulation of the microglia express IGF-1 in the early postnatal SVZ, but IGF-1 is not involved in the action of activated microglia during this period. Sagittal sections were immunostained with anti-CD11b (green: microglia) and anti IGF-1 (red) antibodies. Right panel, Magnified image of the square in the left. A subpopulation of microglia is positive for IGF-1 (arrowheads). The percentage of CD11b+IGF-1+ was 43.42 ± 6.72% in CD11b+ cells. B, Minocycline did not affect the amount of IGF-1 in the early postnatal SVZ. Minocycline was administered by intraperitoneal injection for 3 d beginning at P2 (30 mg/kg/d, P2-P4, n = 6/group), and the amount of IGF-1 in the SVZ was quantified by ELISA. C, Minocycline decreased the amount of inflammatory cytokines in the SVZ. IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, and TNF-α levels in the SVZ tissue lysate were measured by BioPlex cytokine detection assay system. *p < 0.05 (Student's t test). n = 6 rats/group. Data are mean ± SEM. Similar results were obtained in two independent experiments.