Table 1.
Cytosolic calcium dynamics parameters
| 20 μm ATP, spinal cord, P60 |
20 μm ATP, spinal cord, P90 |
20 μm ATP, cortex, P2 |
1 μm FCCP → 20 μm ATP, spinal cord, P60 |
1 μm TG, spinal cord, P60 |
25 μm dantrolene/20 μm ATP, spinal cord, P60 |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT (n = 27) | G93A (n = 29) | WT (n = 48) | G93A (n = 44) | WT (n = 20) | G93A (n = 31) | WT (n = 12) | G93A (n = 13) | WT (n = 19) | G93A (n = 37) | WT (n = 24) | G93A (n = 20) | |
| Peak F/F0 | 3.03 ± 0.21 | 4.34 ± 0.23* | 3.56 ± 0.18 | 6.09 ± 0.49* | 3.24 ± 0.32 | 5.13 ± 0.24* | 3.89 ± 0.38 | 5.34 ± 0.42* | 2.07 ± 0.22 | 3.04 ± 0.24* | 4.19 ± 0.50 | 6.01 ± 0.75 |
| Time-to-peak (s) | 49.9 ± 2.0 | 39.4 ± 2.5* | 34.3 ± 2.6 | 26.5 ± 2.6* | 43 ± 2.4 | 26 ± 0.9* | 44.3 ± 3.2 | 37.8 ± 1.9 | 69.5 ± 7.2 | 66.8 ± 5.5 | 40 ± 5.4 | 25.8 ± 6.2* |
| ½ peak time (s) | 56.7 ± 13.7 | 53.6 ± 5.1 | 40.1 ± 3.2 | 41.9 ± 2.9 | 22 ± 0.5 | 21 ± 1.4 | 36.3 ± 5.8 | 29.4 ± 2.8 | 77.3 ± 13.5 | 84.3 ± 9.7 | n.d. | n.d. |
| Clearance rate | 0.83 ± 0.09 | 1.1 ± 0.09 | 1.3 ± 0.1 | 1.6 ± 0.2 | n.d. | n.d. | n.d. | n.d. | 0.28 ± 0.03 | 0.35 ± 0.02 | n.d. | n.d. |
Parameters of cytosolic calcium changes during ER and mitochondrial calcium release in spinal cord and cortical astrocytes were determined from Fluo4 fluorescence plots (for images and traces, see Fig. 1). See Materials and Methods for determination of each parameter.
*p < 0.05 by Student's t test. Error indicates SEM, and n denotes total number of astrocytes analyzed from at least four coverslips, derived from at least three independent spinal cord preparations. n.d., Not determined.