Figure 1.

Experimental design. Ancestral Φ6 was split into six populations and evolved for 300 generations at high (H1, H2, H3) or low (L1, L2, L3) Multiplicity of Infection (MOI) as described in Montville et al. (2005). These six populations were then evolved for 10 days with a heat shock each day. A single passage consists of heat shocking a diluted viral lysate in soft agar and plating the virus to obtain a plate with ~10,000 plaques. The plates are grown overnight and the virus is then extracted to obtain a high-titer lysate.