Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 5;5:19. doi: 10.3389/fphys.2014.00019

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Smith, Lamattina and Collins.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effects of PTTH and insulin on signaling phosphoproteins. Prothoracic glands from day 2 fifth instar larvae were incubated for 20 min medium with or without insulin (6 uM), followed by 80 min in one of the following treatments: no hormone (Control), insulin (6 uM), PTTH (7 nM), or insulin + PTTH. (TOP) Western blots of cellular lysates, probed for phosphoproteins; (BOTTOM) Ecdysone secretion determined by radioimmunoassay of culture medium at the end of the 80 incubation period. Asterisks denote statistically significant ecdysone secretion relative to no-hormone controls (n = 5 glands per group). Insulin did not enhance secretion, alone or in conjunction with PTTH (ANOVA, Tukey–Kramer post-hoc test, significance p > 0.05).