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. 2014 Feb 5;5:19. doi: 10.3389/fphys.2014.00019

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Copyright © 2014 Smith, Lamattina and Collins.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Bombyx bombyxin II on ecdysone secretion. Prothoracic glands from day 3 fifth instar larvae were incubated for 4 or 8 h in medium containing no hormone (Control) or Bombyx bombyxin II (160 nM) (n = 6 and 11 glands per group, respectively). In separate experiments, glands were incubated for 4 h in no hormone or 160 nM bombyxin, and then challenged for 90 min in medium with no hormone, bombyxin, PTTH (7 nM) or PTTH + bombyxin (n = 16 glands per group). Culture medium was assayed for ecdysone by radioimmunoassay. None of the bombyxin-treated groups differed from their respective controls (ANOVA, Tukey–Kramer post-hoc test, p > 0.05).