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. 2014 Feb 5;4:3977. doi: 10.1038/srep03977

Figure 7. Compound NSC 228155 stimulates in vitro dimerization of sEGFR domain II and not that of sErbB2.

Figure 7

The binding reaction was carried out in the absence of β-mercaptoethanol. Detection of dimeric proteins by Western blotting was carried out using anti-His primary antibody and DyLight 800-labeled secondary antibody (a). The curve shows the percentage of dimers in a total pool of monomeric and dimeric forms of DII/sEGFR detected at different protein-to-compound ratios (b). Sequence alignment between domain II of sEGFR and sErbB2 (c).