Figure 4. Allo-response regulation in iPS-DCregs.

(A) The degree of allo-CD4, CD8 T cell proliferation and proportion of the Foxp3-positive Treg cells among the CD4⁺ T cells, stimulated by the BM-DCcons, BM-DCregs and iPS-DCregs was analyzed. The 2 × 10⁴ cells of iPS-DCregs, BM-DCregs and BM-DCcons were used as stimulator and co-cultured with responder T cells (2 × 10⁵), describing as first experiment in the section of MLR in Materials and Methods. (B) The allo-CD4 and CD8 T cell proliferation stimulated by the mature BM-DCcons was regulated by BM-DCregs and iPS-DCregs, not by BM-DCcons. The proportion of Foxp3-positive cells among CD4 T cells was assessed under conditions stimulated by BM-DCcons and regulated by DCs. The X-irradiated BM-DCcons (1 × 10⁴) were used as stimulator and co-cultured with responder T cells (2 × 10⁵) and the iPS-DCregs, BM-DCregs or BM-DCcons (2 × 10⁴), describing as other experiment in the section of MLR in Materials and Methods. (C) The weights (upper) and cell counts (bottom) of the popliteal lymph nodes obtained from the mice injected with BM-DCcons (1 × 10⁶) only or in combination with 5-×10⁵ of BM-DCcons, BM-DCregs or iPS-DCregs into the footpads. The data are expressed as the mean ± SEM from more than three independent experiments.