Figure 6.

AdipoR1 knockdown does not change isoquercitrin-induced AMPK signaling in H4IIE cells. H4IIE cells were transfected with AdipoR1 siRNA or control siRNA as indicated, followed by isoquercitrin treatment for 12 h. (A) After transfection, AdipoR1 expression was determined by quantitative RT-PCR. (B) Phosphorylation and total AMPKα protein expression were measured by Western blotting. Results were analyzed and shown as means ± SEM (n = 3). #P < 0.05 between isoquercitrin and control plus AdipoR1 siRNA.