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. 2014 Feb 5;5:17. doi: 10.3389/fphys.2014.00017

Figure 1.

Figure 1

DA-3 mammary tumor cells express SEMA7A. (A) Shown is the confocal images of DA-3 mammary tumor cells for SEMA7A expression; (B) SEMA7A expression in EpH4 and DA-3 mammary tumor cells as determined by qPCR; (C) DOT blot analysis of DA-3 mammary tumor cell culture showing that SEMA7A is solubilized; (D) mRNA expression of SEMA7A in peritoneal elicited macrophages (PEMs) from normal and DA-3 mammary tumor-bearing mice; (E) Western blot analysis of total protein from PEMs from control and DA-3 mammary tumor bearers with 7 mice/group; also shown is integrated intensity graph of western blot indicating higher levels of SEMA7A expression in tumor bearers' PEMs. In-vitro experiments are representative of three independent experiments, ***p ≤ 0.001.