Skip to main content
. 2014 Feb 5;5:17. doi: 10.3389/fphys.2014.00017

Figure 4.

Figure 4

Decreased tumor-derived SEMA7A results in reduced in vitro macrophage migration and CXCL2/MIP-2 production. (A) Effect of SEMA7A gene silencing on SEMA7A expression in DA-3 mammary tumor cells as determined by western blot analysis; (B) migration of Calcein AM labeled RAW 264.7 macrophages toward cell-free supernatants from either wild type DA-3 tumor cells, SEMA7A scramble shRNA DA-3 tumor cells, SEMA7A shRNA knockdown DA-3 cells or serum free media alone. (C) mRNA expression of CXCL2/MIP-2 on macrophages from normal mice in vitro treated with conditioned media from either wild type, SEMA7A scramble or SEMA7A knockdown DA-3 mammary tumor cells. In-vitro experiments are representative of three independent experiments. **p ≤ 0.01.