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. 2014 Feb 5;8:18. doi: 10.3389/fncel.2014.00018

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Copyright © 2014 Baj, Patrizio, Montalbano, Sciancalepore and Tongiorgi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of spines and synapses in WT and Mecp2^−/y mouse hippocampal neurons. (A) Proximal and distal fields of WT and Mecp2^−/y mouse apical dendrites. (Scale Bar = 5 μm) (B) Spine density in proximal and distal region of WT (black) and Mecp2^−/y (white) apical dendrites at DIV6, 9,12, 15. (C,D) Spine type percentage with respect to total spine number at different time points (mean + standard errors). (E) Synapse identification by SYN1 (red) and PSD95 (green) signals co-localization (merge) at DIV12 in WT and MeCP2^−/y neurons (Scale bar = 5 μm). (F) Quantitative analysis of SYN/PSD95 co-localization in WT (black) and Mecp2^−/y (white) in proximal (left panel) and distal dendritic fields (right panel). The Y-axis reports the percentages of colocalized PSD95 with respect to total PSD95 signals, while the X-axis reports the different DIV considered. (G) Synapse identification by SYN1 (red) and Gephyrin (green) signals co-localization (merge) at DIV12 in WT and MeCP2^−/y neurons (Scale bar = 5 μm). (H) Quantitative analysis of SYN/ Gephyrin co-localization in WT (black) and Mecp2^−/y (white) in proximal (left panel) and distal dendritic fields (right panel). The Y-axis reports the percentages of colocalized Gephyrin with respect to total Gephyrin signals, while the X-axis reports the different DIV considered. ^**p ≤ 0.01 and ^*p ≤ 0.05 (One-Way ANOVA with Holm-Sidak's pairwise multiple comparison).