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. Author manuscript; available in PMC: 2015 Jan 3.
Published in final edited form as: Angew Chem Int Ed Engl. 2013 Nov 20;53(1):199–204. doi: 10.1002/anie.201307387

Figure 4.

Figure 4

Alpha assay confirms that modification with SML-8-73-1 renders K-Ras G12C biochemically inactive. Untagged GMPPNP-loaded K-Ras G12C was incubated with a 10 fold molar excess GDP (red), SML-8-73-1 (green) or buffer (blue). Under these conditions K-Ras G12C becomes completely labelled by SML-8-73-1 or loaded with GDP or GMPPNP. These K-Ras G12C preparations were then added at the indicated concentrations to compete for binding with Alpha-tagged K-Ras G12C (donor):Raf-RBD (acceptor) complexes which had been pre-formed. SML-8-73-1-bound K-Ras G12C (green) resembles GDP-bound K-Ras G12C (red) with respect to affinity for Raf-RBD.

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