Table 2.
Antibodies used for immunohistochemical detection
| Primary Antibody | Secondary Antibody | ||||||
|---|---|---|---|---|---|---|---|
| Stain | Positive control | Species | Dilution | Supplier | Species | Dilution | Supplier |
| a-SMA | Monkey aorta | Mouse anti-human | 1:60 | Sigma A5691 | Goat anti-mouse | 1:300 | Biorad 170-6516 |
| CD-68 | Monkey lymph node | Mouse anti-human | Pre-dilute | Zymed/Invitrogen 08-0125 | Goat anti-mouse | 1:300 | Biorad 170-6516 |
| MMP-1 | Human ovarian tumor | Rabbit | 1:600 | Fitzgerald 10R-M112a | Rabbit polyclonal | Undiluted | Thermo Shandon TL-060-HL |
| TIMP-1 | Human prostate tumor | Mouse | 1:100 | Fitzgerald 10R-M112b | Rabbit polyclonal | Undiluted | Thermo Shandon TL-060-HL |
| Procollagen -1 | Human skin wound | Rat | 1:600 | Abcam ab64409 | Goat anti-rat | Undiluted | Biocare GHP516 G |
α-SMA indicates alpha-smooth muscle cell actin; CD, clusters of differentiation; MMP-1, matrix metalloproteinase-1; TIMP-1, tissue inhibitor of metalloproteinase-1.