Table 3.

Discrimination of mismatched DNA targets by B4-series and reference ONs.^a

ON	Sequence	B =	DNA: 5′-GTG ABA TGC
			Tm/°C	ΔTm/°C
			T	A	C	G
D2	3′-CAC TAT ACG		27.5	−17.0	−15.5	−9.0
W4	3′-CAC TWT ACG		27.5	−20.0	−17.0	−16.0
X4	3′-CAC TXT ACG		34.0	−16.5	−7.5	−17.0
Y4	3′-CAC TYT ACG		39.0	−21.0	−12.0	−17.0
Z4	3′-CAC TZT ACG		35.0	−21.5	−14.0	−14.5

^aFor conditions of thermal denaturation experiments, see Table 1. T_m’s of fully matched duplexes are shown in bold. ΔT_m = change in T_m relative to fully matched DNA:DNA duplex.